Improved proliferation of early transduced triVSTs. Early transduced (ET) and late transduced (LT) triVST with retrovirus expressing GD2.CAR were co-cultured for 72 hours with (A) GD2-positive LAN-1 neuroblastoma cells, or with (B) autologous LCLs at 4:1 of VST to LCL ratio or (C) Ad5f35-pp65-transduced LCLs (LCL-Adpp65). Proliferation of CD3+ T cells was evaluated by eFluor dilution by flow cytometry. Histograms are representative results from one donor. Cell division indexes were calculated using FlowJo software. Results are shown as mean ± SD, n = 3.