Figure 5

Bladder cancer lesions exposed to IFNα + poly-I:C or BCG + IFNα + poly-I:C show strongly-enhanced CTL-attracting activity. Day 6 effector Granzyme B⁺/CD8⁺ T cells (induced by SEB-loaded LPS + IFNγ-matured DC [36,37]) were harvested and allowed to migrate for 2 hours towards the supernatants from the differentially-treated bladder cancer tissues in 24 trans-well system. Migrated cells in the bottom chamber were harvested and stained for CD8, Granzyme B. The counts of Granzyme B⁺ CD8⁺ T cells were analyzed by FACS. The results were evaluated using two- tailed, paired Student’s t test. Statistically significant differences between groups are highlighted by * (P < 0.05) or ** (P < 0.01).