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Figure 3 | Journal for ImmunoTherapy of Cancer

Figure 3

From: Survival with AGS-003, an autologous dendritic cell–based immunotherapy, in combination with sunitinib in unfavorable risk patients with advanced renal cell carcinoma (RCC): Phase 2 study results

Figure 3

Representative flow cytometry plots detailing the gating strategy to identify CD28+/CD45RA- CD8 + T cells expressing effector molecules. The viable CD3+ CD8+ T cells in the lymphoycte gate were identified and futher subgated to analyze CD28 and CD45RA expression on the Brdu+, IFN-g+ CD107a+ IL-2+ and TNFa+ cells within the CD8+ T cell gate. The number of cellular events in the CD28+/CD45RA- upper left quadrant were used to determined the absolute number of cells/ml. Identical gates were used for all samples analyzed.

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