Phenotype of human lymphocytes recovered from the spleen and tumor of tumor-bearing huPBL-NSG and from primary human prostate cancers. Single cell suspensions were prepared from the spleen and tumor recovered from huPBL-NSG mice at 4 weeks (A) and from a primary human prostate cancer removed at the time of prostatectomy (B). Cells were stained with an antibody cocktail, and then analyzed by flow cytometry for the expression of CD4 vs CD8 (top panel, gated on all cells expressing human CD4 and/or CD8); CD4 vs CD56 (middle panel, gate on all CD3+ cells); and CD4 vs CD69 (bottom panel, gated on all cells expressing human CD4 and/or CD8). The CD8+/CD56+ NKT population is identified by CD4-/CD56+ T cells. Representative experiment, n = 3 experiments.