Figure 4

T- naïve, T-cm and T-em subsets in the spleen and tumor. Single cells were prepared from spleens (A) and tumors (B) recovered from huPBL-NSG mice 3 weeks after implantation and stained with antibodies against human CD3, CD4, CD8, CD45RA and CD127. Cells were gated for expression of CD3 and then analyzed by flow cytometry for the expression of other markers on the CD3+ population. Representative experiment, n = 5 experiments.