Spleen reconstitution and tumor infiltration in NSG animals receiving purified human CD4+ or CD8+ T cells. HuPBL-NSG, huPBL-NSG (−CD8) and huPBL-NSG (−CD4) animals were established as detailed in Figure 5 and implanted with 2x106 PC3 cells by s.c. injection. A & D: The different purified PBL populations, before implantation, were stained with specific antibodies against human CD3, CD4 and CD8 followed by FACS analysis. Results are gated on the human CD3+ cells. Whole spleens B & E and tumors C & F were then recovered after 3 weeks and single cell suspensions stained with specific antibodies against human CD4 and CD8 followed by FACS analysis. Results were gated on all cells expressing CD4 and/or CD8. Representative experiment, n = 3 experiments.