Expression of codon-optimized firefly luciferase in ID8 cells does not alter cellular immune microenvironment
(A) CD3 T cells (y-axis) in ID8 Luc2 (Dark Gray, n = 6) compared to ID8 parental line (White bars, n = 8) in mouse tumor, ascites, and spleen (x-axis) at 12–15 weeks post tumor implant. (B) CD8 T-cell populations, (C) CD4 T-Cell populations, (D) FOXP3 T-regulatory cells populations, (E) CD11bGr1 myeloid derived suppressor cells or MDSC’s. All cell subtypes were CD45+ gated. Horizontal bars are SEM.