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Figure 2 | Journal for ImmunoTherapy of Cancer

Figure 2

From: NK cells and CD8+ T cells cooperate to improve therapeutic responses in melanoma treated with interleukin-2 (IL-2) and CTLA-4 blockade

Figure 2

Combination IL-2 and CTLA-4 blockade immunotherapy increases tumor immune infiltration. (A) Flow cytometry plots of tumors dissected at day 14 and analyzed by flow cytometry for CD45 expression from the experiment described in Figure 1A. Only tumor-infiltrating lymphocytes were analyzed (as determined by comparison of intravenous CD45 staining compared to in vitro CD45 staining). (B) Cumulative graph showing mean percent CD45+ T cell infiltration (of live cells) in the tumor from three independent experiments described in (A). (C) Representative immunofluorescence microscopy images of CD45 (red) and DAPI (4′,6-diamidino-2-phenylindole; blue) staining of tumors from experiment in (A). Scale bars = 20 microns. Three to five mice were included in each group per experiment. *P < 0.05, ***P < 0.001, ns = not significant.

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