Skip to main content
Figure 3 | Journal for ImmunoTherapy of Cancer

Figure 3

From: NK cells and CD8+ T cells cooperate to improve therapeutic responses in melanoma treated with interleukin-2 (IL-2) and CTLA-4 blockade

Figure 3

Combination IL-2 and CTLA-4 blockade immunotherapy increases the proportion of tumor-infiltrating CD8+ T cells. (A) Flow cytometry plots of tumors dissected at day 14 and analyzed for CD3 + CD8+ T cells (of CD45+ cells within the tumor) by flow cytometry from the experiment described in Figure 1A. (B) Cumulative graph showing the mean percent of CD3 + CD8+ T cells from experiment in (A). (C) Cumulative graph showing the mean percent of CD3 + CD8+ T cells in the tumor-draining lymph nodes from experiment in (A). (D) Representative histograms showing expression of cell markers from CD3 + CD8+ T cells in the tumor and from a representative tumor-draining lymph node (LN). Numbers represent mean fluorescence intensity (MFI). (E) Cumulative graph of mean percent of CD3 + CD8+ T cells expressing PD-1 from experiment in (A). Cumulative figures are from at least three independent experiments (with 3–5 mice per group in each experiment). *P < 0.05, ** P < 0.01, ns = not significant.

Back to article page