Figure 4

Combination IL-2 and CTLA-4 blockade immunotherapy increases the proportion of tumor-infiltrating Tregs. (A) Flow cytometry plots of tumors dissected at day 14 and analyzed for regulatory CD4 + Foxp3+ T cells (Tregs; of CD45 + CD3+ cells within the tumor) by flow cytometry from the experiment described in Figure 1A. (B) Cumulative graph showing mean percent of Tregs from experiment in (A). (C) Ratio of CD8+ T cells to Tregs in the tumor from experiment in (A). (D) Cumulative graph showing mean percent of Tregs from the tumor-draining lymph nodes from experiment in (A). (E) Ratio of CD8+ T cells to Tregs in the tumor-draining lymph node from experiment in (A). Cumulative figures are from at least three independent experiments (with 3–5 mice per group in each experiment). *P < 0.05, **P < 0.01, ns = not significant.