Skip to main content
Figure 5 | Journal for ImmunoTherapy of Cancer

Figure 5

From: NK cells and CD8+ T cells cooperate to improve therapeutic responses in melanoma treated with interleukin-2 (IL-2) and CTLA-4 blockade

Figure 5

Combination IL-2 and CTLA-4 blockade immunotherapy increases the proportion and changes the differentiation of tumor-infiltrating NK cells. (A) Flow cytometry plots of tumors dissected at day 14 and analyzed for the proportion of NK cells (of CD45 + CD3- cells within the tumor) by flow cytometry from the experiment described in Figure 1A. (B) Cumulative graph showing mean percent of NK cells from experiment in (A). (C) Cumulative graph showing mean percent of NK cells from the tumor-draining lymph nodes from experiment in (A). (D) Representative histograms showing expression of cell markers by NK cells in the tumor and from a representative tumor-draining lymph node (LN). Numbers represent mean fluorescence intensity (MFI). (E) Flow cytometry plots showing expression of CD27 and CD11b on tumor-infiltrating NK cells from experiment in (A). Cumulative figures are from at least three independent experiments (with 3–5 mice per group in each experiment). *P < 0.05, **P < 0.01, ns = not significant.

Back to article page