Healthy donor CD4+CD25- T cells proliferate and secrete IFN-gamma in response to survivin peptide pools. 1×105 Purified CD4+CD25- cells were stimulated for 6 days with 1 ×104 autologous DCs loaded with CMV peptide pool (DC:CMV) positive control, survivin peptide pool (DC:survivin), or unloaded (DC:null) negative control. Proliferation (A) and IFN-gamma secretion (B) were elicited by DC:survivin, figures represent the mean of three independent experiments from different healthy donors and error bar represents standard error of the mean. For 10 consecutive evaluable healthy donors, a stimulation index was calculated (C) [1×105 CD4+CD25- T cells stimulated with 1×104 DC:survivin (numerator)/Mean of > =10 DC:null stimulated T cell controls (denominator)]. Box and whiskers represents multiple stimulation indices (Line = Mean, Box = 25%-75% CI, Whiskers = minimum and maximum) for each donor. *p < 0.05.