Fig. 4

Immune cell analysis. Mice were divided into treatment groups (no treatment, Cy only or Cy and lysate/CpG) and treated according to the schedule in Fig. 2a. a Spleens were harvested and pooled from 5 mice in each treatment group on days 4, 11 and 22 of the treatment schedule. The depletion and recovery of CD4⁺ T cells, CD8⁺ T cells, CD4⁺FoxP3⁺ T cells, CD4⁺ CD44⁺ CD62L⁻ effector memory (EM) and CD8⁺ CD44⁺ CD62L⁻ EM cells is shown over time. b On day 22, spleens were harvested and pooled from 4 to 5 mice in each treatment group. CD3⁺ CD4⁺ or CD3⁺ CD8⁺ cells were analyzed for intracellular Ki67 expression. c–e Spleens were pooled from 3 to 5 mice in each treatment group. c Absolute number of CD3⁻ CD11c⁺ DCs on day 11 of the treatment schedule in Fig. 2a. d The percentage of spleen CD3⁻ CD11c⁺ dendritic cells differentially expressing CD11b, B220 and CD8α on day 22. e The percentage of CD3⁻CD11c⁺ DC expressing MHC class II (IA^k+) on day 22. Graphs A-E represent data combined from 2 independent experiments. Data was analyzed for statistical significance using one-way ANOVA