Fig. 5
Cy and allogeneic lysate/CpG treatment is effective in regressing AgN2a tumor. a Groups of mice were treated with either Cy and AgN2a lysate/CpG or Cy and 9464D allogeneic lysate/CpG according to the protocol in Fig. 2a. Mice were followed for tumor growth and were euthanized when tumor reached 250 mm2. Nine of 17 AgN2a lysate/CpG and 8 of 15 9464D lysate/CpG treated mice did not have palpable tumor 100 days post tumor inoculation. Data was analyzed for statistical significance using the log-rank (Mantel-Cox) test. The graph represents data combined from 2 separate experiments. The Cy and CpG control group was tested once with 5 mice per group showing statistical significance. b Groups of AgN2a-bearing mice were treated with CpG and (1) irrelevant splenocyte lysate, (2) control 5T33 multiple myeloma tumor lysate, (3) allogeneic 9464D neuroblastoma tumor lysate, or (4) syngeneic AgN2a neuroblastoma tumor cell lysate according to the schedule in Fig. 2a. On day 22 after tumor inoculation, bone marrow was harvested and pooled from 3 mice in each group. CD8 T cells were purified by immunomagnetic cell separation and 105 were incubated overnight with 105 AgN2a stimulator cells. IFN-γ production was analyzed per ELISPOT assay. Background signal (T cells only with no tumor cell stimulators) was subtracted from the experimental values. The graph represents data from 1 to 2 independent experiments. Data was analyzed for statistical significance using the Student’s t test