Fig. 1
From: Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
Overlay image of a triple color Fluorospot well. PBMC were tested for the secretion of IFNÉ£ (FITC spots, green), IL-17A (Cy3, orange), and IL-22 (Cy5, red). The appropriate multi-level evaluation of such sample reveals 7 sub-populations (3 single secretors, 3 dual secretors, 1 triple secretor [11])