Fig. 1

SCNP Process. The SCNP process is coordinated from sample collection through to data analysis and visualization. Sample collection requires standard sample draw into sodium-heparin coated tubes and overnight shipment at ambient temperature. Reagents and methods for the laboratory assay are qualified and controlled for research, GxP or CLIA use as appropriate. The laboratory assay is controlled and multiplexed across 96 well plates to enable broad biological analysis of each donor sample, with normalization across plates, instruments and time. A custom informatics infrastructure ensures reliable and efficient data tracking and maintenance of data integrity. Multiple metrics are established for analysis of signaling magnitude, cell population-based responses and signaling inhibition