Fig. 2

Data analysis. The cloud-based SCNPDataPortal enables investigator analysis of the data set in an interactive manner. Individual web pages present data that address the specific objectives for each study. In the example shown these pages enable the analysis of; disease compared to healthy donor signaling; interrogation of the mechanism of action (MOA) of a candidate molecule or therapeutic; analysis of biomarkers for pharmacodynamic studies, patient selection / stratification and / or toxicity. Visualization options include heatmaps providing an overview of the data and serving as a platform for deeper data mining. Parallel plots provide a means to identify donor subgroups, with each line representing a donor sample with the associated signaling across multiple nodes and cell subsets. In the example shown, two donor subgroups are identified by analysis of signaling in 3 node/cell subset combinations. Box-and-whisker plots enable the comparison of the range of signaling between donor subgroups for selected nodes/cell subsets, with the coloring of each donor data point based upon demographic or clinical criteria. In the example shown, signaling is compared in disease vs. healthy donor samples, with color coding for African American (blue) or caucasian (red) donor samples. Clicking on the individual donor data enables interrogation of the single cell distributions for each data point