Fig. 4

Ability of AdV-transduced DC to induce AFP-specific T cell responses. a and (b) DC from HLA-A2+ HDs (n = 4) were transduced with AdVhAFP or AdVeGFP-AFP at MOI 2000 for 3 hr, then co-cultured with autologous PBMC for 12–13 days. a CD8+ T cells were restimulated with autologous DC (loaded with AFP as in the initial stimulation) for an additional 10d, and then analyzed for AFP-specific intracellular TNF-α production against three immunodominant HLA-A2-restricted peptides. b CD4+ T cells were collected after the initial stimulation and analyzed for AFP-specific intracellular cytokine production. (C and D) DC from HLA-A2+ HCC patients (n = 3–4) were transduced with AdVhAFP or AdVeGFP-AFP at MOI 2000 for 3 hr, then co-cultured with autologous PBMC for 12–13 days. c CD8+ T cells were collected and analyzed for AFP-specific intracellular TNF-α production against three immunodominant HLA-A2-restricted peptides. d CD4+ T cells were collected and analyzed for AFP-specific intracellular cytokine production. For each panel, the solid lines represent the mean value. *, P < 0.05