Fig. 7

DC endocytose AFP primarily via the mannose receptor. a DC were stained for flow cytometric analysis. Red histogram represents endocytic receptor staining; black histogram represents isotype control staining. Data from one representative healthy donor (of three total HD tested) is shown. b DC were pre-treated with inhibitors for 30 min, and co-cultured with fluorescently-labeled nAFP (top panels) or tAFP (bottom panels) for an additional 1 hr. Percent inhibition was calculated based on untreated control cells. Columns, mean of four HD; bars, standard deviation. c and (d) DC were incubated with fluorescently-labeled nAFP for 2 hr, then fixed and stained for MR (c) or CD36 (d). All images are representative of three independent experiments performed and were taken using a 63x objective