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Fig. 8 | Journal for ImmunoTherapy of Cancer

Fig. 8

From: Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma

Fig. 8

Ability of AFP protein-loaded DC to induce AFP-specific T cell responses. a and (b) DC from HLA-A2+ HDs (n = 4) were cultured with nAFP or tAFP (at 10 μg/ml for 2 hr) with or without pre-treatment with MR blocking antibody (at 10 μg/ml for 30 min), matured for an additional 24 h, then co-cultured with autologous PBMC for 12–13 days. a CD8+ T cells were restimulated with autologous DC (loaded with AFP as in the initial stimulation) for an additional 10d, and then analyzed for AFP-specific intracellular TNF-α production against three immunodominant HLA-A2-restricted peptides. b CD4+ T cells were collected after the initial stimulation and analyzed for AFP-specific intracellular cytokine production. c and (d) DC from HLA-A2+ HCC patients (n = 3–4) were cultured with nAFP or tAFP (at 10 μg/ml for 2 hr) with or without pre-treatment with MR blocking antibody (at 10 μg/ml for 30 min), matured for an additional 24 h, then co-cultured with autologous PBMC for 12–13 days. (C) CD8+ T cells were collected and analyzed for AFP-specific intracellular TNF-α production against three immunodominant HLA-A2-restricted peptides. (D) CD4+ T cells were collected and analyzed for AFP-specific intracellular cytokine production. For each panel, the solid lines represent the mean value. *, P < 0.05

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