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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Quantitative real-time PCR assisted cell counting (qPACC) for epigenetic - based immune cell quantification in blood and tissue

Fig. 1

Assay principle – BSC treated genomic DNA specific qPCR. a Only demethylated cytosines in the target cell type (i.e. CD4+ T-cells) convert to uracil during bisulfite conversion (BSC). This allows BSC genomic DNA specific segregating primer and probes to bind and lead to a qPCR product. b Methylated cytosines in non - target cells do not change during BSC treatment of genomic DNA leading to a primer mismatch and no qPCR product

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