Fig. 1From: Quantitative real-time PCR assisted cell counting (qPACC) for epigenetic - based immune cell quantification in blood and tissueAssay principle – BSC treated genomic DNA specific qPCR. a Only demethylated cytosines in the target cell type (i.e. CD4+ T-cells) convert to uracil during bisulfite conversion (BSC). This allows BSC genomic DNA specific segregating primer and probes to bind and lead to a qPCR product. b Methylated cytosines in non - target cells do not change during BSC treatment of genomic DNA leading to a primer mismatch and no qPCR productBack to article page