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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Tasquinimod triggers an early change in the polarization of tumor associated macrophages in the tumor microenvironment

Fig. 2

CD11b+F4/80+ infiltrating cells are functionally skewed from a pro-tumor CD206+ M2 phenotype into a CD206 anti-tumor M1 phenotype after tasquinimod treatment. Modulation of myeloid CD11b+ subpopulations in MC38 tumors by tasquinimod. a Percent CD11b+ cells of infiltrating cells, b representative FACS plots F4/80+CD206+ staining of tumor infiltrating CD11b+ cells, c F4/80+ cells as frequency of total CD11b+ cells, d frequency of CD206+ cells in tumors ± tasquinimod treatment (**p < 0.01; two-way ANOVA, Error bars indicate s.e.m) and e frequency of Ly6ChighLy6G and Ly6G+ cell populations in tumor. f Up-regulation of M1 markers and down-regulation of M2 markers in infiltrating CD11b+F4/80+ after tasquinimod treatment. Tumor infiltrating CD11b+F4/80+ cells were isolated from MC38-C215 tumors at day 14, FACS-sorted and qRT-PCR was performed on the indicated genes. g Infiltrating F4/80+ cells are less immune suppressive after tasquinimod treatment. CD4+ T cells were stimulated with αCD3 and α CD28 in the presence of F4/80+ cells at indicated ratios (F4/80:CD4) and proliferation was measured by 3H-thymidine incorporation (**p < 0.01 and ***p < 0.001; t-test, Error bars indicate s.e.m)

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