Fig. 4

M1 macrophages, induced by tasquinimod modulates the expression of different angiogenic factors. a Median Fluorescence Intensity (MFI)values of CD206, MHC-II and CD86 cell surface expression evaluated by flow cytometry on isolated tumor infiltrating CD11b⁺ cells gated on the F4/80^high population after 1 and 3 days of in vivo exposure to tasquinimod. Results from one out of three representative experiments are shown. b Up-regulation of M1 genes Nos2 (iNos) and Il-12β, and down-regulation of M2 genes CD206, Arg-1 and Il-10 mRNA expression in isolated tumor infiltrating CD11b⁺ cells after 1 and 3 days of in vivo exposure to tasquinimod. c Changes of pro-angiogenic genes Vegfa, Vegfc, Fgf2, Nrp1 and Il-6 mRNA expression in isolated tumor infiltrating CD11b⁺ cells after 1 and 3 days of in vivo exposure to tasquinimod (versus control; * p < 0.05; ** p < 0.01 and *** p < 0.001; One way ANOVA with Dunnetts multiple comparison test, Error bars indicate s.e.m)