Fig. 4
From: Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors
DARPin28z murine and human CAR-T cells are capable of killing HER2+ tumor cells. a Expression of HER2 on a panel of five tumor cell lines was verified by flow cytometry using a primary anti-HER2 antibody followed by a secondary detection antibody (thick lines). Shaded histograms indicate secondary only controls and numbers indicate mean fluorescence intensity. b-e Transduced murine T cells (effectors) were incubated together with tumor cells (targets) at various E:T ratios for 6 h in a 96-well plate. Each E:T was tested in triplicate. Error bars = standard error (SE). f-j Human CAR-T cells were incubated together with tumor cells at various E:T ratios for 6 h. Each E:T was tested in triplicate, data shown is the mean from n = 3 experiments repeated with T cells from two donors. Error bars = SE. Statistics compare scFv28z vs DARPin28z (* = p < 0.05 ** = p < 0.005 *** = p < 0.001)