Table 1 Amino acid sequences of CAR domains. The ten n-terminal and ten c-terminal amino acids which flank the protein regions utilized as CAR domains (with the exception of c-myc for which the sequence is listed in its entirety)

Murine CAR sequences:

Domain

N-terminus

C-terminus

 c-myc tag

EQKLISEEDL

 CD8 hinge

VISNSVMYFS

SVKGTGLDFA

 CD28 transmembrane and cytoplasmic

ALVVVAGVLF

PARDFAAYRP

 CD3ζ cytoplasmic

LRAKFSRSAE

ALHMQTLAPR

Human CAR sequences:

Domain

N-terminus

C-terminus

 CD8 hinge

SALSNSIMYF

GGAVHTRGLD

 CD28 transmembrane and cytoplasmic

FWVLVVVGGV

PPRDFAAYRS

 CD3ζ cytoplasmic

RVKFSRSADA

ALHMQALPPR