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Table 1 Improved euthanasia criteria

From: The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer

Criteria of euthanasia

Liao et al.

Improved criteria

Weight

Cachexia

Loss of 2 g in 48 h

Loss of 3 g in 7 days

Clinical deterioration

Increased respiratory frequency

Increased respiratory frequency

Anorexia

Hunched back with tremor

Ascites

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No spontaneous movement when nudged

  1. Weight and clinical status should be evaluated once every 48 h. The improved criteria allow for more objective criteria for euthanasia of animals