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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Mismatch in epitope specificities between IFNγ inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma

Fig. 6

Immunoproteasome expression in melanoma biopsies. Formalin fixed paraffin embedded human melanoma biopsies from a single patient were scored by a pathologist as having ‘absent’ or ‘brisk’ tumor infiltrating lymphocytes (TILs) present, as labeled. 4 μM sections were co-stained for expression of CD3 to identify TILs, and either of the immunoproteasome subunits LMP2 or LMP7 as indicated. DAPI was used to visualize cell nuclei. Images were acquired using a VECTRA automated quantitative pathology imaging system and analysis was performed using InForm software. Images are shown as pseudo-brightfield IHC (left panels), or multifocal fluorescent images (right panels)

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