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Table 1 Stimulants currently available for the TruCulture® Whole Blood Collection and Culture system

From: Immune monitoring technology primer: immunoprofiling of antigen-stimulated blood

Description of stimulant

Target

Null TruCulture (TC) tube (TC Tubes Null)

Control

Tumor Necrosis factor (TNF)-alpha tube (TC Tubes TNF-α 10 ng/ml)

Cytokine

Intron A/Interferon (IFN)-alpha tube (TC Tubes IFN-α)

Cytokine

Interferon (IFN)-beta tube (TC Tubes IFN-β)

Cytokine

Interleukin (IL)-1beta + TNF-α tube (TC Tubes IL-1β + TNF-α)

Cytokine

Intron A/IFN-α + anti-CD3 + anti-CD28 tube (TC Tubes IFN-α + CD3 + CD28)

Cytokine

Intron A/IFN-α + Lipopolysaccharide (LPS)-EB (Escherichia coli 0111:B4) high concentration tube (TC Tubes IFN-α + LPS-EB)

Cytokine/TLR4 Ligand

Adenosine Triphosphate (ATP) + LPS-EB tube (TC Tubes ATP + LPS-EB)

NLRP3 Inflammasome/TLR4 Ligand

Lauroyl-γ-D-glutamyl-meso-diaminopimelic acid (C12-iE-DAP) tube (TC Tubes C12-iE-DAP)

NOD Ligand

Lipoarabinomannan (LAM) from M. smegmatis tube (TC Tubes LAM-MS)

NOD Ligand

Staphylococcal Enterotoxin B (SEB) tube (TC Tubes SEB)

T-Cell

Anti-CD3/CD28 tube (TC Tubes CD3 + CD28)

T-Cell

SEB/anti-CD28 tube (TC Tubes SEB + CD28)

T-Cell

anti- CD3 tube (TC Tubes CD3)

T-Cell

Zymosan (ZYM) tube (TC Tubes ZYM)

TLR2 Ligand

Fibroblast-stimulating Lipopeptide (FSL) tube (TC Tubes FSL-1)

TLR2 Ligand

Heat killed E. coli 0111:B4 tube (TC Tubes HKEB)

TLR2 Ligand

Heat Killed Lactobacillus rhamnosus tube (TC Tubes HKLR)

TLR2 Ligand

Polyinosinic:polycytidylic acid (Poly I:C) tube (TC Tubes Poly I:C)

TLR3 Ligand

LPS tube (TC Tubes LPS)

TLR4 Ligand

LPS-EB high concentration tube (TC Tubes LPS-EB)

TLR4 Ligand

LPS + SEB tube (TC Tubes LPS + SEB)

TLR4 Ligand/T-Cell

Resiquimod R848 tube (TC Tubes R848)

TLR7/8 Ligand

Gardiquimod (GDQ) tube (TC Tubes GDQ)

TLR7/8 Ligand

Class A CpG oligonucleotide + LPS-EB high concentration tube (TC Tubes ODN2216 + LPS-EB)

TLR9 Ligand