Fig. 4

Cancer stems cells undergo apoptosis following treatment with drozitumab. a) Dissociated tumor cells from 11424, 14244 and 12424 treated in vitro with 10 μg/ml of drozitumab antibody for 1 h and incubated for 7 h with 10 μg/ml of anti-human Fc IgG antibody. Cells were stained for ESA+, CD44+, CD24+ and cleaved caspase-3 and analyzed by flow cytometry. b) Percent of CSCs and bulk tumor cells with cleaved caspase-3. Statistics by Student t-test; **p <0.01. Experiment performed three times