Fig. 5
Treatment of tumor bearing mice in vivo with drozitumab induces apoptosis primarily in triple positive cells. Mice were treated with 200 μg drozitumab and tumors were resected, disaggregated and percentage of cleaved caspase-3 in CSCs vs. bulk tumor cells analyzed by flow cytometry. Representative flow plots and quantification of cleaved caspase-3 in patient xenografts: a & d) 11424, b & e) 14244 and c & f) 12424. n = 3 mice/group. Statistics by Student t-test; *p <0.05, **p <0.01