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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Pancreatic cancer stem cells in patient pancreatic xenografts are sensitive to drozitumab, an agonistic antibody against DR5

Fig. 6

Drozitumab treatment depletes DR5+ cells in vitro. Subsequently, DR5 is increasingly detected on residual cells and reaches pre-treatment levels by 48 h post-treatment in both Panc-1 (a human cell line, a-d) and cells derived from a patient xenograft (12424, e-g). Cells were treated in culture for 10 h with 10 μg of drozitumab and 10 μg of α-human IgG Fc antibody and cells were assessed for DR5 expression immediately after treatment (0 h) or intermittently at 8, 24 and 48 h following treatment. a, e) Plots of expression relative to untreated controls. b, c Representative flow plots from Panc-1. d Fold change of DR5 mRNA in Panc1 cells compared to untreated controls. f, g Representative flow plots from 12424 PDX cells. Statistics by Student t-test; *p <0.05, **p <0.01 compared to untreated controls. Experiment performed twice in triplicate. (Colors in b, c and f, g represent times post-treatment illustrated by bars in a and e respectively)

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