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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Single dose denileukin diftitox does not enhance vaccine-induced T cell responses or effectively deplete Tregs in advanced melanoma: immune monitoring and clinical results of a randomized phase II trial

Fig. 5

Circulating antigen-specific CD8+ T cells in patients treated or untreated with denileukin diftitox. Peripheral blood mononuclear cells were seeded on anti-IFN-γ Ab-coated ELISPOT plates. 5 μM of peptide was then added to each well and incubated overnight. The plates were then incubated with the biotin-labeled anti-IFN-γ Ab and developed according to manufacturer’s procedure. The plates were read and analyzed on CTL-ImmunoSpot S6 Core Analyzer from Cellular Technology Ltd. Each point represents the numerical difference of Ag-specific CD8+ T cells in PBMCs post-vaccine and pre-treatment for each patient. Each point represents an individual patient. Paired student t-test was used to determine whether there was a difference between the pre- and post- samples in each cohort

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