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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Adenosine-generating ovarian cancer cells attract myeloid cells which differentiate into adenosine-generating tumor associated macrophages – a self-amplifying, CD39- and CD73-dependent mechanism for tumor immune escape

Fig. 7

“TAM-like” macrophages suppress CD4+ T cell proliferation via a CD39 and CD73-dependent mechanism. Polarized macrophages were generated in co-culture with OAW-42 OvCA cells whereas M1 macrophages were induced by treatment with LPS and IFN-γ. CD4+ T cells were isolated from healthy volunteers and stained with CFSE before activation with plate-bound anti-CD3 and soluble anti-CD28 antibodies. Activated CFSE+ CD4+ T cells were then co-incubated with M2 macrophages in the absence (T + MФ (Co-culture)) or presence of CD39 or CD73-inhibitors ARL67156 (T + MФ (Co-culture) + ARL) or, respectively, APCP (T + MФ (Co-culture) + APCP), or a combination of both (T + MФ (Co-culture) + ARL + APCP). T cell proliferation was determined by flow cytometry via CFSE dilution, as described previously [7] (n = 3, ** indicates p < 0.01 as assessed by unpaired, two-sided Student’s t-test). While a representative histogram is shown in a, mean values from three independent experiments are depicted in (b)

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