Fig. 1

5-FU treatment promotes B7-H1 protein expression in whole cell lysates from HCT 116 WT ^+/+ and HCT 116 p53 ^−/− colon cancer cell lines. a Western blots from seven independent experiments measuring B7-H1 and actin expression in HCT 116 p53^+/+ and HCT 116 p53^−/− cells. The “X” in experiment # 2 indicates a skipped lane when loading the samples. Unt = untreated control, 0.5, 5, 50 and 500 = 5FU doses in uM, IFN = 10 ng/ul of interferon gamma. b Quantification of B7-H1 expression using ImageJ. Data is represented as mean B7-H1 band intensity/mean actin band intensity +/− SEM. *** p < 0.001 relative to the untreated control