Fig. 4

5-FU treatment promotes B7-H1 protein expression in a dose dependent manner in OE33 esophageal adenocarcinoma cells. a Western blots from three independent experiments measuring B7-H1 and actin expression. Unt = untreated control, 0.5, 5, 50 and 500 = doses in uM of 5FU, IFN = 10 ng/ul of interferon gamma. b Quantification of B7-H1 expression using ImageJ. Data is represented as mean PD-L1 band intensity/mean actin band intensity +/− SEM. *** p < 0.001 relative to the untreated control