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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Expansion of tumor-infiltrating lymphocytes (TIL) from human pancreatic tumors

Fig. 4

Stimulated pancreatic TIL display effector function. a Propagated TIL were stimulated with 25 ng/mL of PMA and 500 nM ionomycin for 18 h, then stained for CD3, CD4, CD8, and CD107a or the corresponding isotype antibodies followed by intracellular staining for IFN-γ and analyzed by flow cytometry. All cells stained with the isotype control were stimulated under the same conditions. Representative FACS plots gated on CD3+ CD8+ TIL are shown. b Scatter plots display the percentage of each indicated population for individual patient data. Each analysis refers to the percentage of IFN-gamma (IFN-g) + and/or CD107a + cells within the CD4 or CD8 gates of the CD3+ lymphocytes. Error bars represent mean % ± SD (n = 12)

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