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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Expansion of tumor-infiltrating lymphocytes (TIL) from human pancreatic tumors

Fig. 5

CD8+ pancreatic TIL maintain effector function after selective expansion. a CD8+ T cells were enriched through magnetic depletion of CD4+ cells from bulk TIL and subject to the rapid expansion protocol (REP). b Representative FACS plots of CD3+ TIL were analyzed for CD4 and CD8 expression before and after the procedure described in a. c CD8-enriched TIL were then stimulated with 25 ng/mL of PMA and 500 nM ionomycin and analyzed for surface CD107a and intracellular IFN-gamma (IFN-g) by flow cytometry

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