Fig. 5

CD8⁺ pancreatic TIL maintain effector function after selective expansion. a CD8⁺ T cells were enriched through magnetic depletion of CD4⁺ cells from bulk TIL and subject to the rapid expansion protocol (REP). b Representative FACS plots of CD3⁺ TIL were analyzed for CD4 and CD8 expression before and after the procedure described in a. c CD8-enriched TIL were then stimulated with 25 ng/mL of PMA and 500 nM ionomycin and analyzed for surface CD107a and intracellular IFN-gamma (IFN-g) by flow cytometry