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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Anti-PD-1 increases the clonality and activity of tumor infiltrating antigen specific T cells induced by a potent immune therapy consisting of vaccine and metronomic cyclophosphamide

Fig. 5

Expression of cytotoxic genes in tumour tissue after treatment with DPX vaccination, mCPA and anti-PD-1 by RT-qPCR. Mice were implanted with C3 tumors and treated with 1 week of mCPA commencing 14 days after implantation. Mice were vaccinated on study day 21 and treated with anti-PD-1 or isotype control on study day 26. All mice were terminated on study day 31. Total tumor mRNA analysed for gene expression by RT-qPCR, results normalized to the level of GAPDH mRNA and presented as fold of increase in mRNA level over the untreated control that was arbitrary set as 1. a Cd8a (CD8α), b Gzmb (Granzyme B), c Ifng (IFN-γ), d Prf (Perforin), e Tbx21 (T-bet), f Cd4 (CD4), g Pdcd1 (PD-1), h Cd274 (PD-L1), i Gata3 (GATA-3). Results pooled from three separate experiments, n = 2–12, statistics between indicated groups by 1-way ANOVA followed by LSD post-test: *p < 0.05, **p < 0.01, ***p < 0.001

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