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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Anti-PD-1 increases the clonality and activity of tumor infiltrating antigen specific T cells induced by a potent immune therapy consisting of vaccine and metronomic cyclophosphamide

Fig. 6

Anti-PD-1 treatment increases the clonality of TILs induced by DPX vaccination and mCPA. Mice were implanted with C3 tumors and treated with 1 week of mCPA commencing 14 days after implantation. Mice were vaccinated on study day 21 and treated with anti-PD-1 or isotype control on study day 26. All mice were terminated on study day 31. Total gDNA was isolated from tumors for TCRβ sequencing. a Total productive TCRβ molecules in each sample, b Clonality, c Cumulative frequency (Σ Freq.) of top 10 most frequent clones. Results from a single experiment, n = 5, statistics between indicated groups by 1-way ANOVA followed by LSD post-test: *p < 0.05

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