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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Anti-PD-1 increases the clonality and activity of tumor infiltrating antigen specific T cells induced by a potent immune therapy consisting of vaccine and metronomic cyclophosphamide

Fig. 7

Anti-PD-1 increases the diversity and frequency of antigen-specific clones induced by DPX vaccination and mCPA. A R9F-clone library was created by purifying the R9F-specific CD8α + T cells clones from the spleens of vaccinated, C3 tumor bearing mice (n = 3) using FACS and performing TCRβ sequencing. From the three mice, 26 different clones were identified at a frequency >1 % and considered R9F-specific. These clones were screened for in tumor TCRβ data generated in Fig. 6. a Number of different R9F-specific clones from the library identified in each treatment group b Frequency of R9F-specific clones

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