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Table 4 Recommended standard materials for immune assays

From: Validation of biomarkers to predict response to immunotherapy in cancer: Volume I — pre-analytical and analytical validation

Technology/Assay Recommended Reference Materials
Blood-based assays Cell lines or control PBMC donor samples prepared and cryopreserved following SOP (reference samples)
Immunohistochemistry assays Control cell lines or tissue specimens (e.g., human tonsil for PD-L1 staining) or cell lines processed and embedded in paraffin (not to be used for interpreting patient data)
RNA/DNA based assays (NGS, TCR/BCR sequencing, gene expression profiling) Synthetic mimic libraries, TCR/BCR DNA synthetic templates, synthetic vectors, company or WHO and NIST references