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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Fusion of the dendritic cell-targeting chemokine MIP3α to melanoma antigen Gp100 in a therapeutic DNA vaccine significantly enhances immunogenicity and survival in a mouse melanoma model

Fig. 6

Vaccine-specific CD8+ T-cell tumor infiltrate analysis. Vaccinations occurred on days 3 and 10 post challenge. Mice were sacrificed on day 17 and lymphocyte-enriched tumor suspensions were collected. CD8+ TILs reactive to ex vivo stimulation by gp10025-33 peptide were delineated by Intracellular Cytokine Staining Flow Cytometry measuring cytoplasmic IFN-γ accumulation post stimulation. a Percentage of CD8+ TILs reactive to antigen. b Estimated total number of reactive CD8+ TILs normalized to tumor size. All groups were significantly different from each other by ANOVA except for the comparison of PBS to MIP3α-CSP. HA irrelevant negative peptide and PMA/ionomycin positive controls confirmed the protocol validity (data not shown). Data are from one of two representative experiments with 3–5 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001

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