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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation

Fig. 2

Vγ2Vδ2 T cells expanded by pulse zoledronate stimulation with IL-2 exhibit slightly increased degranulation and expression of granzyme B and perforin compared with those expanded by continuous stimulation when exposed to pamidronate-treated PC-3 cancer cells. Human PBMC were cultured either continuously with zoledronate (5 μM) or pulsed with zoledronate (100 μM) for 4 h and then washed twice before culture with IL-2 (1000 IU/ml). After 14 d, expanded Vγ2Vδ2 T cells were purified by positive selection or left unpurified. PC-3 cancer cells were treated overnight with pamidronate (200 μM) and then washed. Pamidronate-treated PC-3 cells were incubated with unpurified or purified Vγ2Vδ2 T cells for 4 h in duplicate samples followed by surface and intracellular mAb staining. CD107a, IFN-γ, granzyme B, perforin, IL-17A, and IL-21 levels on or in Vγ2Vδ2 T cells were assessed by flow cytometric analysis. Mean ± SD is shown. Representative of two experiments. *p < 0.05 compared to 10 μM zoledronate continuous stimulation using the unpaired t-test

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