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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation

Fig. 5

Expanding Vγ2Vδ2 T cells by pulse zoledronate stimulation with IL-15 increased their purity and numbers compared with continuous stimulation. a Expansion of Vγ2Vδ2 T cells in response to varying doses of HMBPP and zoledronate with IL-15. Human Vγ2Vδ2 T cells were expanded ex vivo from PBMC by exposure to HMBPP (left panels) or zoledronate (right panels). Human PBMC were cultured in 96-well plates either continuously with varying starting concentrations of HMBPP or zoledronate (open circles) or pulsed with varying concentrations of zoledronate for 4 h (closed circles) followed by washing twice. IL-15 was added to 100 ng/ml on day 3. Thereafter, media was changed every 2–3 d depending on cell growth. On day 14, Vγ2Vδ2 T cell numbers were determined by flow cytometric analysis. Results for 8 donors are shown. b, upper panel Purity of Vδ2 T cells under the indicated conditions with IL-15 (100 ng/ml) after 14-day culture in 24-well plates. Each point represents one donor (n = 8). ** p = 0.003, Mann–Whitney U test. Lower panel, Number of Vδ2 T cells under the indicated conditions with IL-15 (100 ng/ml) after 14-day culture in 24-well plates. Each point represents one donor (n = 8). ** p = 0.007, Mann–Whitney U test. c Comparison of the purity (left panel) and number (right panel) of Vδ2 T cells expanded by continuous (10 μM) or pulse (100 μM) stimulation with zoledronate with IL-15 in 24-well plates. Each line connects the results for an individual donor from (b)

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