Analyses of the peripheral immunome following multiple administrations of avelumab, a human IgG1 anti-PD-L1 monoclonal antibody

Donahue, Renee N.; Lepone, Lauren M.; Grenga, Italia; Jochems, Caroline; Fantini, Massimo; Madan, Ravi A.; Heery, Christopher R.; Gulley, James L.; Schlom, Jeffrey

doi:10.1186/s40425-017-0220-y

Table 1 Complete list of 123 peripheral immune cell subsets analyzed by flow cytometry

From: Analyses of the peripheral immunome following multiple administrations of avelumab, a human IgG1 anti-PD-L1 monoclonal antibody

1. Total CD4⁺ T cells • PD-L1⁺ CD4 • PD-1⁺ CD4 • EOMES⁺ CD4 • TCR⁺ CD4 • Tbet⁺ CD4 • BATF⁺ CD4 • CTLA-4⁺ CD4 • Tim-3⁺ CD4 • ICOS⁺ CD4 o PD-L1⁺ ICOS⁺ CD4 o PD-1⁺ ICOS⁺ CD4 • Total naïve (CCR7⁺CD45RA⁺) CD4 o PD-L1⁺ naïve CD4 o PD-1⁺ naïve CD4 o CTLA-4⁺ naïve CD4 o Tim-3⁺ naïve CD4 • Total central memory (CCR7⁺ CD45RA^-) CD4 o PD-L1⁺ CM CD4 o PD-1⁺ CM CD4 o CTLA-4⁺ CM CD4 o Tim-3⁺ CM CD4 • Total effector memory (CCR7^- CD45RA^-) CD4 o PD-L1⁺ EM CD4 o PD-1⁺ EM CD4 o CTLA-4⁺ EM CD4 o Tim-3⁺ EM CD4 • Total EMRA (CCR7^-CD45RA⁺) CD4 o PD-L1⁺ EMRA CD4 o PD-1⁺ EMRA CD4 o CTLA-4⁺ EMRA CD4 o Tim-3⁺ EMRA CD4 2. Total CD8⁺ T cells • PD-L1⁺ CD8 • PD-1⁺ CD8 • EOMES⁺ CD8 • TCR⁺ CD8 • Tbet⁺ CD8 • BATF⁺ CD8 • CTLA-4⁺ CD8 • Tim-3⁺ CD8 • Total naïve (CCR7⁺CD45RA⁺) CD8 o PD-L1⁺ naïve CD8 o PD-1⁺ naïve CD8 o CTLA-4⁺ naïve CD8 o Tim-3⁺ naïve CD8	• Total central memory (CCR7⁺CD45RA^-) CD8 o PD-L1⁺ CM CD8 o PD-1⁺ CM CD8 o CTLA-4⁺ CM CD8 o Tim-3⁺ CM CD8 • Total effector memory (CCR7^- CD45RA^-) CD8 o PD-L1⁺ EM CD8 o PD-1⁺ EM CD8 o CTLA-4⁺ EM CD8 o Tim-3⁺ EM CD8 • Total EMRA (CCR7^-CD45RA⁺) CD8 o PD-L1⁺ EMRA CD8 o PD-1⁺ EMRA CD8 o CTLA-4⁺ EMRA CD8 o Tim-3⁺ EMRA CD8 3. Total Tregs • PD-L1⁺ Tregs • PD-1⁺ Tregs • CTLA-4⁺ Tregs • ICOS⁺ Tregs • CD45RA⁺ Tregs • CD49d^- Tregs 4. Total B cells • PD-L1⁺ B cells • PD-1⁺ B cells • CTLA-4⁺ B cells • Tim-3⁺ B cells 5. Total NK • PD-L1⁺ NK • PD-1⁺ NK • Tim-3⁺ NK • Total mature (CD16⁺ CD56^dim) NK o PD-L1⁺ mature NK o PD-1⁺ mature NK o Tim-3⁺ mature NK • Total functional intermediate (CD16⁺ CD56^br) NK o PD-L1⁺ functional intermediate NK o PD-1⁺ functional intermediate NK o Tim-3⁺ functional intermediate NK	• Total immature (CD16^- CD56^br) NK o PD-L1⁺ immature NK o PD-1⁺ immature NK o Tim-3⁺ immature NK • Total unconventional (CD16^- CD56^dim) NK o PD-L1⁺ unconventional NK o PD-1⁺ unconventional NK o Tim-3⁺ unconventional NK 6. Total NK-T • PD-L1⁺ NK-T • PD-1⁺ NK-T • Tim-3⁺ NK-T 7. Total cDC • PD-L1⁺ cDC • PD-1⁺ cDC • CD83⁺ cDC • Tim-3⁺ cDC 8. Total pDC • PD-L1⁺ pDC • PD-1⁺ pDC • CD83⁺ pDC • Tim-3⁺ pDC 9. Total MDSC • PD-L1⁺ MDSC • PD-1⁺ MDSC • CD16⁺ MDSC • Total monocytic (CD14⁺ CD15^-) MDSC o PD-L1⁺ mMDSC o PD-1⁺ mMDSC o CD16⁺ mMDSC • Total granulocytic (CD14^- CD15⁺) MDSC o PD-L1⁺ gMDSC o PD-1⁺ gMDSC o CD16⁺ gMDSC • Total lineage negative (CD14^- CD15^-) MDSC o PD-L1⁺ lin neg MDSC o PD-1⁺ lin neg MDSC o CD16⁺ lin neg MDSC

Nine classic subsets were identified as well as 114 refined subsets relating to maturation and function within the classic subsets (Lepone et al., ref. [15])
BATF basic leucine zipper transcription factor ATF-like, cDC conventional dendritic cells, CM central memory, CTLA-4 cytotoxic T lymphocyte-associated protein-4, EM effector memory, EMRA terminally differentiated effector memory, EOMES eomesodermin, gMDSC granulocytic MDSC; ICOS inducible T cell co-stimulator, lin neg MDSC lineage negative MDSC, MDSC myeloid derived suppressor cell, mMDSC monocytic MDSC, NK natural killer, pDC plasmacytoid DC, PD-1 programmed cell death protein 1, PD-L1 programmed cell death ligand-1, Tbet T box expressed in T cells, TCR T cell receptor, Tim-3 T cell immunoglobulin and mucin domain-3, Tregs regulatory T cells

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