Fig. 2

CART72 cell growth curves. Individual CART72 cell growth curves from trials C-9701 and C-9702 are shown. Lymphapheresis products were depleted of red blood cells and stimulated with CD3xCD28 beads on Day 1 in the presence of IL-2 (200 IU/mL) with removal of beads on Day 3. Spinoculation transduction with the CC49-ζ vector was performed on Days 5 and 7 followed by continued cell culture in serum-free media supplemented with IL-2 (200 IU/mL). Minimum harvest criteria of 3 x 10¹⁰ total T cells was achieved on all lots by Day 17