Fig. 3

Akt inhibition promotes the generation of memory CD19CAR T cells from different T cell subsets. a Bulk T cells, purified TCM, and purified naïve/memory T cells were transduced with lentivirus encoding second generation CD19CAR vector and expanded in a medium containing 50U/L rhIL2, in the presence and absence of 1 μM Akt inhibitor for 17–21 days. Resultant CD19CAR T cells were stained with biotinylated Erbitux (cetuximab), followed by streptavidin-PE for CAR detection and antibodies against CD62L. Percentages of CAR + CD62L+ cells are depicted on the basis of the gating of isotype-stained cells. b Percentages of CD62L + CD28+ T cells after gating on CAR + CD8+ from six lines derived from two different donors are presented. c Mean percentages of immunoreactive cells + SEMs from six lines of two different donors are presented