Skip to main content
Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Ex vivo Akt inhibition promotes the generation of potent CD19CAR T cells for adoptive immunotherapy

Fig. 4

Akt inhibition does not dampen cell-mediated cytotoxicity but increases cytokine production of CD8 + CD19CAR T cells. After 21 days of in vitro expansion, a CD8 + CD19CAR T-cells with and without Akt inhibitor treatment were co-cultured with LCLs at a 1:1 ratio in medium containing Golgi plug and CD107a for six hours at 37 °C. KG1a cells were used as negative stimulator and LCL OKT3 as positive stimulator. Degranulation was determined using multicolor flow cytometry. Percentages of CAR + CD107a + T cells are presented. b Accumulated data from three different donors are presented. c CD8 + CD19CAR T cells with and without Akt inhibitor treatment were co-cultured with LCLs at a 1:1 ratio in medium for eighteen hours at 37 °C. Supernatants were harvested and cytokines were measured by a cytometric bead array. Means + SEMs of triplicates are depicted. KG1a cells were used as a negative stimulator and LCLOKT3 as positive stimulator

Back to article page