Skip to main content
Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Ex vivo Akt inhibition promotes the generation of potent CD19CAR T cells for adoptive immunotherapy

Fig. 5

Akti-treated CD8 + CD19CAR T cells exhibit increased anti-tumor efficacy in vivo. 0.5 × 106 acute lymphoid leukemic SupB15 cells engineered with GFP firefly luciferase (GFPffluc+) were inoculated into NSG mice intravenously (i.v) on day -5. After confirmation of tumor engraftment, 2 × 106 expanded CD8 + CD19CAR+ T cells were adoptively transferred into tumor-bearing mice intravenously. Non-transduced mock cells from the same donor were used as controls. a Tumor signals were monitored by biophotonic tumor imaging and b the bioluminescence signal was measured as total photon flux normalized for exposure time and surface area and expressed in units of photons (p) per second per cm2 per steradian (sr). c Kaplan-Meier survival curve. N = 4 mice per group

Back to article page