Fig. 4

Immunophenotypic differences in sedentary versus active individuals at baseline samples. Immunophenotypes were measured by flow cytometry from baseline samples from 10 active subjects and 5 sedentary subjects. a CD45RO⁺ and PD-1⁺ cells were measured as a percent of parent CD4⁺ or CD8⁺ T cells in sedentary and active subjects. All subjects were included on the %CD45RO⁺ versus %PD-1⁺ plot. b Representative dot plots showing the distribution of positive and negative T cells for CD45RO and PD-1 (CD4 T cells on top, CD8 T cells on bottom.) The graph shows the percent positive PD-1 cells on memory T cells (CD45RO⁺) or naïve T cells (CD45RO⁻) for both CD4 and CD8. ** = p < 0.001 by a paired T test